Combining the characteristics of various existing object motion acceleration measurement methods, a low-cost and convenient motion acceleration measurement method based on MEMS motion sensor is proposed. The method uses multi-sensor data fusion technology to solve the motion posture of the object in real time, and then extracts the motion acceleration of the object from the measured data of the MEMS motion sensor. The feasibility of the method is verified by the MEMS motion sensor based acceleration measurement system. The experimental results show that the acceleration solution error of the method is 0.03 m/s2 at rest, which is basically consistent with the acquisition accuracy of MEMS accelerometer. The acceleration solution error of the method is 0.07 m/s2 when free-floating motion. The coupled motion acceleration will reduce the accuracy of the solution when the object is moving for a long time, but it still has high accuracy in short-time motion.