Aiming at the application of highspeed data transmission, a system is designed to realize PCIE interface based on Xilinx FPGA. The system uses the PCIE hard core integrated on FPGA, develops the PCIE device driver using WinDriver under Windows 7 operation system, caches the data using DDR3 SDRAM and realizes highspeed data transferring with Direct Memory Access(DMA) between a host PC and a Xilinx FPGA board using Verilog hardware description language. The system has been verified on Xilinx VC707 development board and the result shows that the highspeed transmission system based on lane 1 PCIE interface can reach 154 MB/s throughput in DMA read and 169 MB/s throughput in DMA write for a DMA transfer size of 8 MB. The system can satisfy the requirements of reliability and efficiency during data transmission.