Steadystate visual evoked potential (SSVEP) is widely used in brain interface and medical clinical diagnosis when the lock is locked and the signaltonoise ratio and transmission rate are higher. The interaction between auditory and visual stimuli has great influence on brain cognition and its application. Therefore, it is very meaningful to study the effect of auditory stimulation on SSVEP. At the frequency of 12 Hz, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 95% of the light stimulation frequency, respectively, add 500, 1 000, 1 500Hz sine pure tone, loudness of 50 dB of auditory stimulation, the study of SSVEP changes in the law. The results showed that SSVEP showed a “window” effect with the change of duty cycle in the same subjects, and the effect of auditory stimulation on SSVEP was enhanced or inhibited. In addition, the position, number, and degree of enhancement or inhibition of the “window” of the auditory stimulus impact on SSVEP is also due to the fact that SSVEP is enhanced or inhibited. The results provide a meaningful experimental basis for better study of the mechanism of interaction between brain and brain signals produced by visual and auditory stimuli.