Aiming at the complexity of current DA conversion and its conditioning circuit of multichannel signal source, combined with the requirements for analog signal sources , a multichannel analog signal source based on FPGA and PCI bus is designed. The board takes FPGA as the system controller, PCI9054 as PCI bus protocol chip, 100V analog voltage is generated by boost chip lm5022, and the output is controlled by aqy277a Optical MOS Relay of NAIS company; 23 channels of 0-28v adjustable analog voltage are converted by 6 four channel DA conversion chip ad5504. Finally, the calibration coefficient of the upper computer is used to improve the accuracy of output voltage. The results show that the board can output the voltage reliably, and the stability is high, and meets the design requirements.