The virtual binocular vision test is based on a high-speed camera and two planar mirrors to realize the 3D reconstruction of objects, and it solves the problems of synchronization and high cost in traditional binocular vision test. The internal and external parameters of camera calibration are an important guarantee of test precision. In order to solve the issue of human intervention and the complicated process during the calibration, a kind of automatic calibration system of high-speed camera parameter is designed based on the virtual binocular vision and Zhang’s calibration method. The external trigger circuit is designed to control the automatic rotation of the calibration board mounted on the platform bracket so as to realize automatic image shooting of different angle calibration board. The internal and external parameters of the high speed camera are obtained through the automatic processing of images by the open source computer vision library. Finally, the accuracy and practicability of the automatic calibration system of high speed camera are verified, It could provide the basis for subsequent 3D reconstruction and dynamic 3D testing.