Aiming at the existing problems of the need to calibrate instrument hardware before instrument delivery,presents a gain analysis and storage system of conditioning circuit.This system adopts the Agilent 34410A as measurement tool,with the STM32 as the main control chip,with STM32 builtin NAND Flash chip as a storage unit. Slave computer Communicates with PC through USB protocol.Testing results indicate that the system can achieve the function of using PC control the Agilent 34410A to collect a conditioning circuit of each amplifier and attenuator in actual input and output voltage,then calculates their actual gain, last send the collected data to the Flash of STM32.The software of PC reads out the data in the memory via USB,and using the value for subsequent calibration work.