To improve the power generation ability of PVDF piezoelectric film, a PVDF piezoelectric energy collection structure based on vortex-induced vibration mechanism was explored, which converts wind energy into electric energy for micro power electronic products. A signal conditioning circuit was designed to convert the charge output of piezoelectric film into voltage output. The power generation performance of single PVDF piezoelectric film, double and three PVDF piezoelectric films in series and in parallel under different wind speeds was studied by micro wind tunnel experiment. Experimental results show that the resonant frequency of the selected PVDF piezoelectric film is stable at about 50 Hz. The signal conditioning circuit can filter out the high-frequency noise interference effectively, and the output voltage of the signal conditioning circuit increases with the increase of wind speed. The parallel connection of PVDF piezoelectric films and matching load at the end of the circuit can effectively improve the load power. The load power of 36 mW can be achieved when three piezoelectric films are connected in parallel and a 1.25 Ω load is connected at the end of the signal conditioning circuit.