With the development of 5g、WiFi communication technology and the wide application of ultra wideband (UWB) high precision positioning system, the mutual interference between orthogonal frequency division multiplexing (OFDM) signal and UWB signal cannot be ignored in indoor space. In this paper, the analysis method of system equivalent carrier to noise ratio is adopted, combined with the physical characteristics of the two systems, and the physical layer model of the two systems is built by MATLAB. According to the spectral separation coefficient, the amount of noise caused by the interference signal entering the receiver is calculated, and then the BER of OFDM communication system and the positioning accuracy of UWB system are obtained by the equivalent signal to noise ratio of the receiver in the case of interference. Simulation results show that there is signal interference between OFDM signal and UWB signal. The positioning error of UWB signal at the interference center is amplified by more than 12 times, and the bit error rate of OFDM communication signal rises to about 10-3. In other words, the same frequency mutual interference of signals will degrade the performance of 5G, WiFi and other communication systems and seriously affect the positioning accuracy of UWB system. Through the proposed system deployment optimization scheme based on distributed low-power micro-base station, the positioning performance is significantly improved in the case of almost constant communication performance in the experimental area, and the decimeter positioning accuracy can be guaranteed in the whole experimental area. The research results can provide reference for the integrated design and equipment deployment of 5G communication system and UWB positioning system.